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mouse monoclonal antibody against cellular fibronectin  (Millipore)


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    Structured Review

    Millipore mouse monoclonal antibody against cellular fibronectin
    (A–D) <t>Fibronectin</t> predominantly appeared along the laser incision site or lenticular interface. The expression was reduced over time after either ReLEx or refractive lenticule re-implantation. (E–H) Tenascin was absent along the flap interface on week 8 and 16 following ReLEx, but was present along the borders of the stromal lenticule after re-implantation. The intensity of the staining was attenuated over time. (I–L) Collagen type I was expressed uniformly in the full thickness of corneal stroma. No significant anomaly in collagen arrangement was observed in the corneas post-ReLEx and post-reimplantation. (M–P) CD18-positive cells were not seen in all post-operative corneas. Unoperated corneas were used as control. Arrowheads indicate the location of the laser incision site or lenticular interface. PR: post-ReLEx, PLR: post-lenticule re-implantation. Scale bar: 50 µm.
    Mouse Monoclonal Antibody Against Cellular Fibronectin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+antibody+against+cellular+fibronectin/anti+fibronectin/pmc03691223-93-12-18
    Average 90 stars, based on 1 article reviews
    mouse monoclonal antibody against cellular fibronectin - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Reversible Femtosecond Laser-Assisted Myopia Correction: A Non-Human Primate Study of Lenticule Re-Implantation after Refractive Lenticule Extraction"

    Article Title: Reversible Femtosecond Laser-Assisted Myopia Correction: A Non-Human Primate Study of Lenticule Re-Implantation after Refractive Lenticule Extraction

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0067058

    (A–D) Fibronectin predominantly appeared along the laser incision site or lenticular interface. The expression was reduced over time after either ReLEx or refractive lenticule re-implantation. (E–H) Tenascin was absent along the flap interface on week 8 and 16 following ReLEx, but was present along the borders of the stromal lenticule after re-implantation. The intensity of the staining was attenuated over time. (I–L) Collagen type I was expressed uniformly in the full thickness of corneal stroma. No significant anomaly in collagen arrangement was observed in the corneas post-ReLEx and post-reimplantation. (M–P) CD18-positive cells were not seen in all post-operative corneas. Unoperated corneas were used as control. Arrowheads indicate the location of the laser incision site or lenticular interface. PR: post-ReLEx, PLR: post-lenticule re-implantation. Scale bar: 50 µm.
    Figure Legend Snippet: (A–D) Fibronectin predominantly appeared along the laser incision site or lenticular interface. The expression was reduced over time after either ReLEx or refractive lenticule re-implantation. (E–H) Tenascin was absent along the flap interface on week 8 and 16 following ReLEx, but was present along the borders of the stromal lenticule after re-implantation. The intensity of the staining was attenuated over time. (I–L) Collagen type I was expressed uniformly in the full thickness of corneal stroma. No significant anomaly in collagen arrangement was observed in the corneas post-ReLEx and post-reimplantation. (M–P) CD18-positive cells were not seen in all post-operative corneas. Unoperated corneas were used as control. Arrowheads indicate the location of the laser incision site or lenticular interface. PR: post-ReLEx, PLR: post-lenticule re-implantation. Scale bar: 50 µm.

    Techniques Used: Expressing, Staining

    Related Articles

    Incubation:

    Article Title: Refractive lenticule re-implantation after myopic ReLEx: a feasibility study of stromal restoration after refractive surgery in a rabbit model.
    Article Snippet: METHODS.. ReLEx [ 6.00 diopter (D) correction] was performed on six New Zealand White rabbits in one eye.. Each extracted RL was tagged and orientated before storage at 808C for 28 days.

    Article Title: Hyperopic refractive correction by LASIK, SMILE or lenticule reimplantation in a non-human primate model
    Article Snippet: Immunohistochemical staining was performed with sections fixed with 4% paraformaldehyde (Sigma) for 15 minutes, then washed with 1X PBS and blocked in 1X PBS with 0.15% Saponin (Sigma), 0.1% bovine serum albumin (Sigma) and 1.5% Normal Goat Serum Invitrogen) for 10 minutes. .. The sections were subsequently incubated with the following primary antibodies diluted in primary antibody diluent (1X PBS, 0.425% saponin, 1% BSA, 1.5% NGS, 0.0015% tween-20, 0.0015% triton-X): mouse monoclonal antibody against cellular fibronectin (Millipore, Billerica, Massachusetts, U.S.A.) diluted 1: 100; tenascin-C (Abcam, Cambridge, U.K.) diluted 1: 200; mouse monoclonal antibody against heatshock protein 47 (Hsp47) (Enzo Lifesciences, Switzerland) diluted 1:200; collagen type I (Sigma) diluted 1: 100; and CD18 (Novus Biologicals, Littleton, Colorado, U.S.A.) diluted 1: 100 in the blocking solution. .. After washing with 1X PBS, the sections were incubated with goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) at room temperature for 1 hour.

    Article Title: Retreatment strategies following Small Incision Lenticule Extraction (SMILE): In vivo tissue responses
    Article Snippet: .. Cryosections (8 μm in thickness) of the rabbit corneas were fixed with 4% paraformaldehyde (Sigma, St. Louis, MI) for 15 minutes, washed with 0.01M PBS (1st Base, Singapore), blocked with 4% bovine serum albumin (Sigma) in 0.01M PBS, 0.15% Triton X-100 (Sigma) for 1 hour, and incubated with either mouse monoclonal antibody against cellular fibronectin (Millipore) diluted at 1:400, mouse monoclonal antibody against CD11b (BD Pharmingen) diluted at 1:100 in blocking solution, or with pre-diluted mouse monoclonal antibody against Ki-67 (Invitrogen, Carlsbad, CA) at 4 ° C overnight. .. After washing with 0.01M PBS, the sections were incubated with goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) at room temperature for 1 hour.

    Blocking Assay:

    Article Title: Refractive lenticule re-implantation after myopic ReLEx: a feasibility study of stromal restoration after refractive surgery in a rabbit model.
    Article Snippet: METHODS.. ReLEx [ 6.00 diopter (D) correction] was performed on six New Zealand White rabbits in one eye.. Each extracted RL was tagged and orientated before storage at 808C for 28 days.

    Article Title: Hyperopic refractive correction by LASIK, SMILE or lenticule reimplantation in a non-human primate model
    Article Snippet: Immunohistochemical staining was performed with sections fixed with 4% paraformaldehyde (Sigma) for 15 minutes, then washed with 1X PBS and blocked in 1X PBS with 0.15% Saponin (Sigma), 0.1% bovine serum albumin (Sigma) and 1.5% Normal Goat Serum Invitrogen) for 10 minutes. .. The sections were subsequently incubated with the following primary antibodies diluted in primary antibody diluent (1X PBS, 0.425% saponin, 1% BSA, 1.5% NGS, 0.0015% tween-20, 0.0015% triton-X): mouse monoclonal antibody against cellular fibronectin (Millipore, Billerica, Massachusetts, U.S.A.) diluted 1: 100; tenascin-C (Abcam, Cambridge, U.K.) diluted 1: 200; mouse monoclonal antibody against heatshock protein 47 (Hsp47) (Enzo Lifesciences, Switzerland) diluted 1:200; collagen type I (Sigma) diluted 1: 100; and CD18 (Novus Biologicals, Littleton, Colorado, U.S.A.) diluted 1: 100 in the blocking solution. .. After washing with 1X PBS, the sections were incubated with goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) at room temperature for 1 hour.

    Article Title: Reversible Femtosecond Laser-Assisted Myopia Correction: A Non-Human Primate Study of Lenticule Re-Implantation after Refractive Lenticule Extraction
    Article Snippet: .. The following primary antibodies and the corresponding working dilution factor were used: mouse monoclonal antibody against cellular fibronectin (Millipore, Billerica, MA) diluted 1∶400; tenascin-C (Abcam, Cambridge, UK) diluted 1∶200; collagen type I (Sigma) diluted 1∶100; and CD18 (Novus Biologicals, Littleton, CO) diluted 1∶100 in the blocking solution. .. After washing with 1X PBS, the sections were incubated with goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) at room temperature for 1 hour.

    Article Title: Retreatment strategies following Small Incision Lenticule Extraction (SMILE): In vivo tissue responses
    Article Snippet: .. Cryosections (8 μm in thickness) of the rabbit corneas were fixed with 4% paraformaldehyde (Sigma, St. Louis, MI) for 15 minutes, washed with 0.01M PBS (1st Base, Singapore), blocked with 4% bovine serum albumin (Sigma) in 0.01M PBS, 0.15% Triton X-100 (Sigma) for 1 hour, and incubated with either mouse monoclonal antibody against cellular fibronectin (Millipore) diluted at 1:400, mouse monoclonal antibody against CD11b (BD Pharmingen) diluted at 1:100 in blocking solution, or with pre-diluted mouse monoclonal antibody against Ki-67 (Invitrogen, Carlsbad, CA) at 4 ° C overnight. .. After washing with 0.01M PBS, the sections were incubated with goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) at room temperature for 1 hour.

    Next-Generation Sequencing:

    Article Title: Hyperopic refractive correction by LASIK, SMILE or lenticule reimplantation in a non-human primate model
    Article Snippet: Immunohistochemical staining was performed with sections fixed with 4% paraformaldehyde (Sigma) for 15 minutes, then washed with 1X PBS and blocked in 1X PBS with 0.15% Saponin (Sigma), 0.1% bovine serum albumin (Sigma) and 1.5% Normal Goat Serum Invitrogen) for 10 minutes. .. The sections were subsequently incubated with the following primary antibodies diluted in primary antibody diluent (1X PBS, 0.425% saponin, 1% BSA, 1.5% NGS, 0.0015% tween-20, 0.0015% triton-X): mouse monoclonal antibody against cellular fibronectin (Millipore, Billerica, Massachusetts, U.S.A.) diluted 1: 100; tenascin-C (Abcam, Cambridge, U.K.) diluted 1: 200; mouse monoclonal antibody against heatshock protein 47 (Hsp47) (Enzo Lifesciences, Switzerland) diluted 1:200; collagen type I (Sigma) diluted 1: 100; and CD18 (Novus Biologicals, Littleton, Colorado, U.S.A.) diluted 1: 100 in the blocking solution. .. After washing with 1X PBS, the sections were incubated with goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) at room temperature for 1 hour.



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    Millipore mouse monoclonal antibody against cellular fibronectin
    (A–D) <t>Fibronectin</t> predominantly appeared along the laser incision site or lenticular interface. The expression was reduced over time after either ReLEx or refractive lenticule re-implantation. (E–H) Tenascin was absent along the flap interface on week 8 and 16 following ReLEx, but was present along the borders of the stromal lenticule after re-implantation. The intensity of the staining was attenuated over time. (I–L) Collagen type I was expressed uniformly in the full thickness of corneal stroma. No significant anomaly in collagen arrangement was observed in the corneas post-ReLEx and post-reimplantation. (M–P) CD18-positive cells were not seen in all post-operative corneas. Unoperated corneas were used as control. Arrowheads indicate the location of the laser incision site or lenticular interface. PR: post-ReLEx, PLR: post-lenticule re-implantation. Scale bar: 50 µm.
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    Millipore mouse monoclonal anti-cellular antibody against fibronectin
    (A–D) <t>Fibronectin</t> predominantly appeared along the laser incision site or lenticular interface. The expression was reduced over time after either ReLEx or refractive lenticule re-implantation. (E–H) Tenascin was absent along the flap interface on week 8 and 16 following ReLEx, but was present along the borders of the stromal lenticule after re-implantation. The intensity of the staining was attenuated over time. (I–L) Collagen type I was expressed uniformly in the full thickness of corneal stroma. No significant anomaly in collagen arrangement was observed in the corneas post-ReLEx and post-reimplantation. (M–P) CD18-positive cells were not seen in all post-operative corneas. Unoperated corneas were used as control. Arrowheads indicate the location of the laser incision site or lenticular interface. PR: post-ReLEx, PLR: post-lenticule re-implantation. Scale bar: 50 µm.
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    Image Search Results


    (A–D) Fibronectin predominantly appeared along the laser incision site or lenticular interface. The expression was reduced over time after either ReLEx or refractive lenticule re-implantation. (E–H) Tenascin was absent along the flap interface on week 8 and 16 following ReLEx, but was present along the borders of the stromal lenticule after re-implantation. The intensity of the staining was attenuated over time. (I–L) Collagen type I was expressed uniformly in the full thickness of corneal stroma. No significant anomaly in collagen arrangement was observed in the corneas post-ReLEx and post-reimplantation. (M–P) CD18-positive cells were not seen in all post-operative corneas. Unoperated corneas were used as control. Arrowheads indicate the location of the laser incision site or lenticular interface. PR: post-ReLEx, PLR: post-lenticule re-implantation. Scale bar: 50 µm.

    Journal: PLoS ONE

    Article Title: Reversible Femtosecond Laser-Assisted Myopia Correction: A Non-Human Primate Study of Lenticule Re-Implantation after Refractive Lenticule Extraction

    doi: 10.1371/journal.pone.0067058

    Figure Lengend Snippet: (A–D) Fibronectin predominantly appeared along the laser incision site or lenticular interface. The expression was reduced over time after either ReLEx or refractive lenticule re-implantation. (E–H) Tenascin was absent along the flap interface on week 8 and 16 following ReLEx, but was present along the borders of the stromal lenticule after re-implantation. The intensity of the staining was attenuated over time. (I–L) Collagen type I was expressed uniformly in the full thickness of corneal stroma. No significant anomaly in collagen arrangement was observed in the corneas post-ReLEx and post-reimplantation. (M–P) CD18-positive cells were not seen in all post-operative corneas. Unoperated corneas were used as control. Arrowheads indicate the location of the laser incision site or lenticular interface. PR: post-ReLEx, PLR: post-lenticule re-implantation. Scale bar: 50 µm.

    Article Snippet: The following primary antibodies and the corresponding working dilution factor were used: mouse monoclonal antibody against cellular fibronectin (Millipore, Billerica, MA) diluted 1∶400; tenascin-C (Abcam, Cambridge, UK) diluted 1∶200; collagen type I (Sigma) diluted 1∶100; and CD18 (Novus Biologicals, Littleton, CO) diluted 1∶100 in the blocking solution.

    Techniques: Expressing, Staining